Adeno Associated Virus (AAV) is the main vector for gene therapy. Scalable, cost-efficient, and robust filtration and chromatography-based processes are required for purification of AAV. Key for a successful process are high overall yields and efficient impurity removal with maximized empty capsid product impurity removal. Here, we present results from purification process development of AAV2 and AAV5 serotypes, including harvest by cell lysis, clarification, concentration and buffer exchange, affinity capture and finally anion exchange polishing to reduce the empty capsid product impurity. We present a Biacore™ surface plasmon resonance (SPR) assay for robust and accurate determination of AAV2 titer.
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